• Overview
• Nanoflow HPLC »
  • Autosampler Injection
  • Nanoflow HPLC »
    • RP Separation (1D)
    • Switching
    • Coupling LC to MS
• Mass Spectrometry »
  • Introduction »
    • Spectrum
    • Chromatogram
    • Isotopes, Mass Resolution, Mass Accuracy
    • m/z, Charge State
  • Peptide Ionisation »
    • Electrospray
    • MALDI
  • Mass Spectrometry Modes »
    • MS/MS Modes
    • Hybrid Modes
  • Peptide Fragmentation »
    • Overview
    • Collisional Activation Methods
    • Mobile Proton Model

Liquid chromatography coupled online to a tandem mass spectrometer (LC-MS/MS) is a analytical technique used to determine the identity or structure of constituent molecules from within a complex heterogeneous sample.

Liquid chromatography allows for the separation and concentration of analytes (peptides) prior to analysis by a mass spectrometer. This has two clear benefits when compared to an-alysis by mass spectrometry without prior separation. Firstly it allows the mass spectrometer sufficient time to characterise the structure of individual analytes and secondly it allows for a better sensitivity of detection for individual analytes. In the following sections we will look at each these techniques in detail.