Welcome to the Nucleolar Proteome Database. This site contains all of the data from our ongoing proteomic analysis of human nucleoli, which is carried out as a collaboration between the Lamond and Mann laboratories. Using high sensitivity mass spectrometry and stringent criteria, we have so far identified ~700 human nucleolar proteins. More recently, we used a quantitative proteomic approach for the temporal characterization of protein flux through the nucleolus, determining the kinetics of up to 489 nucleolar proteins after various drug treatments and demonstrating that there is no unique, complete proteome for the nucleolus, but rather an overlapping set of proteomes that are relevant to different cell states or conditions.

The data can be searched in a variety of ways (see below). To find out more about the nucleolus, our various proteomics studies, the SILAC method of quantitative proteomics or the structure of this database, follow the appropriate links. For a downloadable PDF file listing all of the proteins alphabetized by name, click here. The same table is available with the proteins sorted by molecular weight by clicking here.

If you require any further information please feel free to contact us directly.

For Citation:

"NoPdb: Nucleolar Proteome Database" (2006) Leung, A.K., Trinkle-Mulcahy, L., Lam, Y.W., Andersen, J.S., Mann, M. and Lamond, A.I. Nucleic Acids Res. 34:D218-20, 2006.