Splicing & snRNPs

Nuclear/Cytoplasmic transport

Nuclear organization

Other research articles

Reviews & Methods

Splicing & snRNPs (Back to top)

"Mass Spectrometry and EST-database searching allows characterization of the multi-protein spliceosome complex"; Neubauer, G., King, A., Rappsilber, J., Calvio, C., Watson, M., Ajuh, P., Sleeman, J., Lamond, A. and Mann, M. (1998) Nature Genetics 20, pp 46-50.

"Dynamic interactions between splicing snRNPs, coiled bodies and nucleoli revealed using snRNP protein fusions to the green fluorescent protein"; Sleeman, J., Lyon, C.E., Platani, M., Kreivi, J.-P. and Lamond, A.I. (1998) Experimental Cell Research 243, pp 290-304.

"Cis-acting elements distinct from the 5' splice site promote U1-independent pre-mRNA splicing"; Crispino, J.D., Mermoud, J.E., Lamond, A.I. and Sharp, P.A. (1996) RNA 2, pp 664-673.

"Identification of hnRNP P2 as TLS/FUS using Electrospray Mass Spectrometry"; Calvio, C., Neubauer, G., Mann, M. and Lamond, A.I. (1995) RNA 1, pp 724-733.

"Influenza virus NS1 protein alters the subnuclear localization of cellular splicing components"; Fortes, P., Lamond, A.I. and Ort’n, J. (1995) J. General Virol. 76, pp 1001-1007.

"Dynamic organization of splicing factors in adenovirus infected cells"; Bridge, E., Xia, D-X., Carmo-Fonseca, M., Cardinali, B., Lamond, A.I. and Pettersson, U. (1995) J. Virol. 69, pp 281-290. "

Regulation of Mammalian Spliceosome Assembly by a Protein Dephosphorylation Mechanism"; Mermoud, J.E., Cohen, P.T.W. and Lamond, A.I. (1994) EMBO J 13, pp 5679-5688.

"Protein Phosphatase 1 can modulate alternative 5' splice site selection in a HeLa splicing extract"; Cardinali, B., Cohen, P.T.W. and Lamond, A.I. (1994) FEBS Lett. 352, pp 276-280.

"Differential interaction of splicing snRNPs with coiled bodies and interchromatin granules during mitosis and assembly of daughter cell nuclei"; Ferreira, J., Carmo-Fonseca, M. and Lamond, A.I. (1994) J. Cell Biology. 126, pp 11-23.

"Novel Solid Phase Synthesis of Branched Oligoribonucleotides, including a Substrate for the RNA Debranching Enzyme"; Sproat, B.S., Beijer, B., Gr¿tli, M., Ryder, U., Morand, K.L. and Lamond, A.I. (1994) J. Chem. Soc., Perkin Transactions 1. pp 419-432.

"Nuclear Organisation of Splicing snRNPs During Differentiation of MEL cells in vitro"; Antoniou, M., Carmo-Fonseca, M., Ferreira, J. and Lamond, A.I. (1993) J. Cell Biology. 123, pp 1055-1068.

"Nuclear Organization of Splicing Small Nuclear Ribonucleoproteins in Adenovirus-Infected Cells" Bridge, E., Carmo-Fonseca, M., Lamond, A.I. and Pettersson, U. (1993) J. Virol. 67, pp 5792-5802.

"An HSV-1 immediate early gene product, IE63, regulates nuclear snRNP distribution" Phelan, A., Carmo-Fonseca, M., McLauchlan, J., Lamond, A.I. and Clements, J.B. (1993) Proc. Natl. Acad. Sci. USA. 90, pp9056-9060.

"Interaction of the human autoantigen p150 with splicing snRNPs"; Blencowe, B.J., Carmo-Fonseca, M., Behrens, S-E., LŸhrmann, R. and Lamond, A.I. (1993) J. Cell Science 105, pp685-697.

"Assembly of snRNP-containing coiled bodies is regulated in interphase and mitosis - evidence that the coiled body is a kinetic nuclear structure"; Carmo-Fonseca, M., Ferreira, J. and Lamond, A.I. (1993) J. Cell Biology. 120, pp841-852.

"Ser/Thr-Specific Protein Phosphatases are Required for both Catalytic Steps of Pre-mRNA Splicing"; Mermoud, J.E., Cohen, P. and Lamond, A.I. (1992) Nucl. Acids Res. 20, pp5263-5269.

"Antisense probes targeted to an internal domain in U2 snRNP specifically inhibit the second step of pre-mRNA splicing"; Barabino, S.M.L., Sproat, B.S. and Lamond, A.I. (1992) Nucl. Acids Res. 20, pp4457-4464.

"Cloning and Intracellular Localization of the U2 snRNP Auxiliary Factor Small Subunit"; Zhang, M., Zamore, P.D., Carmo-Fonseca, M., Lamond, A.I. and Green, M.R. (1992) Proc. Natl. Acad. Sci. USA 89, pp8769-8773.

"Transcription-dependent colocalization of the U1, U2, U4/U6 and U5 snRNPs in coiled bodies"; Carmo-Fonseca, M., Pepperkok, R., Carvalho, M.T. and Lamond, A.I. (1992) J. Cell Biology. 117, pp1-14.

"Nuclease resistant ribozymes with high catalytic activity"; Paolella, G., Sproat, B.S. and Lamond, A.I. (1992) EMBO J. 11, pp1913-1919. ÒSer/Thr-specific protein phosphatases are required for both catalytic steps of pre-mRNA splicingÓ; Mermoud, J.E., Cohen, P. and Lamond, A.I. (1992) Nucl. Acids Res. 20, pp 5263-5269.

"Antisense probes targeted to an internal domain in U2 snRNP specifically inhibit the second step of pre-mRNA splicing"; Barabino, S.M.L., Sproat, B.S. and Lamond, A.I. (1992) Nucl. Acids Res. 20 pp 4457-4464.

"Cloning and intracellular localization of the U2 snRNP auxilliary factor small subunit"; Zhang, M., Zamore, P.D., Carmo-Fonseca, M., Lamond, A.I. and Green, M.R. (1992) Proc. Natl. Acad. Sci USA 89, pp 8769-8773.

"Transcription-dependent colocalization of the U1, U2, U4/U6 and U5 snRNPs in coiled bodies"; Carmo-Fonseca, M., Pepperkok. R., Carvalho, M.T. and Lamond, A.I. (1992) J. Cell Biology 117, pp 1-14.

"Nuclease resistant ribozymes with high catalytic activity"; Paolella, G., Sproat, B.S. and Lamond, A.I. (1992) EMBO J. 11, pp 1913-1919.

"2'-O-Alkyloligoribonucleotides: synthesis and applications in studying RNA splicing"; Sproat, B.S., Iribarren, A., Beijer, B., Pieles, U. and Lamond, A.I. (1991) Nucleosides and Nucleotides 10, pp 25-36.

"Antisense probes containing 2-aminoadenosine allow efficient depletion of U5 snRNP from HeLa splicing extracts"; Lamm, G.M., Blencowe, B.J., Sproat, B.S., Iribarren, A.M., Ryder, U. and Lamond, A.I. (1991) Nucl. Acids Res. 19, pp 3193-3198.

ÒIn vivo detection of snRNP-rich foci in the nuclei of mammalian cellsÓ; Carmo-Fonseca, M., Pepperkok, R., Sproat, B.S., Ansorge, W., Swanson, M. and Lamond, A.I. (1991) EMBO J. 10, pp 1863-1873.

"Site-directed ribose methylation identifies 2'-OH groups in polyadenylation substrates critical for AAUAAA recognition and poly(A) addition"; Bardwell, V.J., Wickens, M., Bienroth, S., Keller, W., Sproat, B.S. and Lamond, A.I. (1991) Cell 65, pp 125-133.

"Mammalian nuclei contain foci which are highly enriched in components of the pre-mRNA splicing machinery"; Carmo-Fonseca, M., Tollervey, D., Pepperkok, R., Barabino, S.M.L., Merdes, A., Brunner, C., Zamore, P., Green, M., Hurt, E. and Lamond, A.I. (1991) EMBO J. 10, pp 195-206.

"Sequence-specific affinity selection of mammalian splicing complexes"; Ryder, U., Sproat, B.S. and Lamond A.I. (1990) Nucl. Acids Res. 18 pp 7373-7379.

"Synthesis and applications of oligoribonucleotides with selected 2'-O-methylation using the 2'-O-[1-(2-fluorophenyl)-4-mythyoxypiperidin-4-yl] protecting group"; Beijer, B., Sulston, I., Sproat, B.S., Rider, P., Lamond, A.I. and Neuner, P. (1990) Nucl. Acids Res. 18 pp 5143-5151.

"Targeted snRNP depletion reveals an additional role for mammalian U1 snRNP in spliceosome assembly"; Barabino, S.M.L., Blencowe, B.J., Ryder, U., Sproat, B.S. and Lamond, A.I. (199) Cell 63 pp 293-302.

"2Õ-O-Alkyloligoribonucleotides as antisense probes"; Iribarren, A.M., Sproat, B.S., Neuner, P., Suston, I., Ryder, U. and Lamond, A.I. (1990) Proc. Natl. Acad. Sci. USA 87, pp 7747-7751.

"Mapping U2 snRNP:pre-mRNA interactions using biotinylated oligonucleotides made of 2'-OMe RNA"; Barabino, S., Sproat, B.S., Ryder, U., Blencowe, B.J. and Lamond, A.I. (1989) EMBO J. 8, pp 4171-4178.

"Antisense probing the human U4/U6 snRNP with biotinylated 2'-OMe RNA oligonucleotides"; Blencowe, B.J., Sproat, B.S., Ryder, U., Barabino, S. and Lamond, A.I. (1989) Cell 59, pp 531-539.

"Probing the structure and function of U2 snRNP with antisense oligonucleotides made of 2'-OMe RNA"; Lamond, A.I., Sproat, B., Ryder, U. and Hamm, J. (1989) Cell 58, pp 383-390.

"Highly efficient chemical synthesis of 2'-O-methyloligoribonucleotides and tetrabiotinylated derivatives; novel probes that are resistant to degradation by RNA or DNA specific nucleases"; Sproat, B.S., Lamond, A.I., Beijer, B., Neuner, P. and Ryder, U. (1989) Nucl. Acids Res. 17, pp 3373-3386.

Nuclear/Cytoplasmic Transport (Back to top)

"Cloning and characterization of hSRP1g, a tissue-specific nuclear transport factor"; Nachury, M.V., Ryder, U.W., Lamond, A.I. and Weis, K. (1998) Proc. Natl. Acad. Sci. USA 95, pp 582-587.

"Characterization of the nuclear protein import mechanism using Ran mutants with altered nucleotide binding specificities"; Weis, K., Dingwall, C. and Lamond, A.I. (1996) EMBO J 15, pp 7120-7128.

"The conserved amino terminal domain of hSRP1a is essential for nuclear protein import"; Weis, K., Ryder, U. and Lamond, A.I. (1996) EMBO J. 15, pp 1818-1825.

"Identification of hSRP1a as a functional receptor for nuclear localisation sequences"; Weis, K., Mattaj, I.W. and Lamond, A.I. (1995) Science 268, pp 1049-1053.

Nuclear Organization ( Back to top)

"Purification and characterisation of p99, a nuclear modulator of protein phosphatase 1 activity"; Kreivi, J.-P., Trinkle-Mulcahy, L., Lyon, C.E., Morrice, N.A., Cohen, P. and Lamond, A.I. (1997) FEBS Lett. 420, pp 57-62.

"Inhibition of protein dephosphorylation results in the accumulation of splicing snRNPs and coiled bodies within the nucleolus"; Lyon, C., Bohmann, K., Sleeman, J. and Lamond, A.I. (1997) Exp. Cell Res. 230, pp 84-93.

"Spatial organization of large scale chromatin domains in the nucleus: a magnified view of single chromosome territories"; Ferreira, J., Paolella, G., Ramos, C. and Lamond, A.I. (1997) J. Cell Biol. 139, pp 1-14.

"The Dynamics of Coiled Bodies in the Nucleus of Adenovirus-infected Cells"; Rebelo, L., Almeida, F., Ramos, C., Bohmann, K., Lamond, A.I. and Carmo-Fonseca, M. (1996) Mol. Biol. Cell 7, pp 1137-1151.

"Neuronal Differentiation in the Hippocampus Involves a Stage-Specific Reorganisation of Subnuclear Structure Both In Vivo and In Vitro"; Santama N., Dotti, C. and Lamond, A.I. (1996) Eur. J. Neuroscience. 8, pp 892-905.

"Mutational analysis of p80 coilin indicates a functional interaction between coiled bodies and the nucleolus"; Bohmann, K., Ferreira, J.A. and Lamond, A.I. (1995) J. Cell Biol. 131, pp 817-831.

"p80 Coilin" Carmo-Fonseca, M., Bohmann, K., Carvalho, M.T. and Lamond, A.I. (1994) Manual of Biological Markers of Disease, B 8.2, pp 1-11.

"Retinoic Acid Regulates Aberrant Nuclear Localization of PML-RARa in Acute Promyelocytic Leukaemia Cells" Weis, K., Rambaud, S., Carvalho, T., Carmo-Fonseca, M., Lavau, C., Jansen, J., Lamond, A.I. and Dejean, A. (1994) Cell. 76, pp 345-356.

Other Research Articles ( Back to top)

"p72: a human nuclear DEAD-box protein highly related to p68"; Lamm, G.M., Nicol, S.M., Fuller-Pace, F.V. and Lamond, A.I. (1996) Nucl. Acids Res. 24, pp 3739-3747.

"ERC-55 is a novel EF-hand protein retained in the ER by a C-terminal HDEL motif"; Weis, K., Griffiths, G. and Lamond, A.I. (1994) J. Biol. Chem. 269, pp 19142-19150.

"Catalytic RNA and the origin of genetic systems"; Lamond, A.I. and Gibson, T.J. (1990) TIG 6, pp 145-149.

"Metabolic complexity in the RNA world and implications for the origin of protein synthesis"; Gibson, T.J. and Lamond, A.I. (1990) J. Mol. Evol. 30, pp 7-15.

Reviews & Methods ( Back to top)

"Purification and Depletion of RNP Particles by Antisense Affinity Chromatography"; Blencow, B.J. and Lamond, A.I. (1998) Methods in Mol. Biol. in press.

"Structure and Function in the Nucleus"; Lamond, A.I. and Earnshaw, W.C. (1998) Science 280, pp 547-553.

"Cell biology and the genome projects - a concerted strategy for characterizing multiprotein complexes by using mass spectrometryÓ Lamond, A.I. and Mann, M. (1997) Exp. Cell Res. 230, pp 84-93.

"From Transcript to Protein" (Meeting Review); Dreyfuss, G., Hentze, M. and Lamond, A.I.(1996) Cell 85, pp 963-972.

"Unexpected Spliceosome Diversity"; Kreivi, J-P. and Lamond, A.I. (1996) Current Biology 6, pp 802-805.

"Wilms' tumour - the splicing connection?"; Lamond, A.I. (1995) Current Biology 5, pp 862-865. "Pre-mRNA Processing"; (1995) R.G. Landes Company (A.I. Lamond ed.)

"Isolation and characterisation of ribonucleoprotein complexes"; Lamond, A.I. and Sproat, B.S. (1994) in "RNA Processing - A Practical Approach" IRL Press at Oxford University Press (D. Hames and S. Higgins, editors) pp 103-140.

"Uncovering the role of Ser/Thr protein phosphorylation in nuclear pre-mRNA splicing"; Mermoud, J.E., Calvio, C. and Lamond, A.I. (1994) Adv. Prot. Phosphatases. 8, pp 99-118.

"The Spliceosome" Lamond, A.I. (1993) Bioessays. 15, pp 595-603. "Localisation of splicing snRNPs in mammalian cells" Lamond A.I. and Carmo-Fonseca, M. (1993) Mol. Biol. Rep.. 18, pp127-133.

"The Coiled Body"; Lamond, A.I. and Carmo-Fonseca, M. (1993) Trends in Cell Biology. 3, pp198-204. "Antisense oligonucleotides made of 2'-O-Alkyl RNA: Their properties and applications in RNA biochemistry" Lamond, A.I. and Sproat, B.S. (1993) FEBS Letters 325, pp123-127.

"2'-O-Alkyloligoribonucleotides"; Sproat, B.S. and Lamond, A.I. (1993) in "Antisense Research and Applications " CRC Press (S.T. Crooke and B. Lebleu, eds.) pp351-362.

"A glimpse into the spliceosome"; Lamond, A.I. (1993) Current Biology. 3, pp62-64. "Non-snRNP Protein Splicing Factors"; Lamm, G.M. and Lamond, A.I. (1993) Biochimica et Biophysica Acta 1173, pp247-265.

"2'-O-Alkyloligoribonucleotides: Probes for studying the Biochemistry and Cell Biology of RNA Processing"; Lamond, A.I. (1993) Biochemical Society Transactions 21, pp1-8.

"Splicing in Stereo" Lamond, A.I. (1993) Nature. 365, pp 294-295.

"2'-O-Methyloligoribonucleotides: synthesis and applications"; Sproat, B.S. and Lamond, A.I. (1991) in "Oligonucleotides and Analogues" IRL Press at Oxford University Press (F. Eckstein, editor) pp 49-86.

ÒASF/SF2 - A splice site selector?"; Lamond, A.I. (1991) TIBS 16, pp 452-453. ÒNuclear RNA ProcessingÓ; Lamond, A.I. (1991) Critical Opinion in Cell Biology 3, pp 493-501.

"2Õ-O-Alkyloligoribonucleotides: synthesis and applications in molecular biology"; Sproat, B.S., Lamond, A.I., Garcia, R.G., Beijer, B., Pieles, U., Douglas, M., Bohmann, K., Carmo-Fonseca, M., Weston, S. and OÕLoughlin, S. (1991) Nucl. Acids Symposium Series No 24, pp 59-62.

"The trimethylguanosine cap is a nuclear targeting signal for snRNPs"; Lamond, A.I. (1990) TIBS 15, pp 451-452.

"Studying pre-mRNA splicing using antisense 2'-OMe RNA oligonucleotides"; Lamond, A.I., Barabino, S., Blencowe, B.J., Sproat, B. and Ryder, U. (1990) Mol. Biol. Rep. 14, p 201.

"Tetrahymena telomerase contains an internal RNA template"; Lamond, A.I. (1989) TIBS 14 pp 202-204.

"The mammalian pre-mRNA splicing apparatus"; Lamond, A.I., Barabino, S. and Blencowe, B.J. (1989) in ÒNucleic Acids & Molecular BiologyÓ vol. 4 (Springer Verlag) pp 243-257.

"RNA editing and the mysterious undercover genes of trypanosomatid mitochondria"; Lamond, A.I. (1988) TIBS 13, pp 283-28..

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